The relative abundance of empty and full AAV capsids in gene therapy vector preparations is a critical quality attribute. A variety of methods are available to perform these measurements including SEC-MALS, which combines separation by size-exclusion chromatography with detection by multi-angle light scattering, UV/Vis, and differential refractometry. However, there are relatively few reports that compare these methods. This webinar compares the outcomes of numerous orthogonal characterization methods on a single vector preparation. We find that SEC-MALS not only provides favorable performance for quality control and product development applications, but that it is uniquely situated to be established as a platform method for the semi-quantitative measurement of empty/full ratios across AAV serotypes, transgenes, and manufacturing processes.
Key Learning Objectives:
How SEC-MALS quantifies empty and full AAVs, as well as other attributes
Are empty/full readouts from different assays comparable?
Other light scattering techniques that quantify AAVs
Who Should Attend:
CMC teams supporting AAV-based therapies
Scientists involved in AAV characterization, analytical method development, process development, and quality control
Lab managers that need to select and support an optimal set of AAV characterization methods
Brought to you by:
Margaret Butko Associate Director, Protein Analytical Characterization at Adverum