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How to calculate the molarity of a buffer?

I have given the concentration of L-Histidine (FW: 155.155g/mol) at 0.24g/L and concentration of Histidine HCl Monohydrate (FW:169.6g/mol) at 3.87g/L. How do I calculate the molarity of buffer? 

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Re: How to calculate the molarity of a buffer?

To calculate the molarity of a buffer solution, you need to follow these steps:

Convert mass to moles:

For L-Histidine:

moles of L-Histidine= molar massmass/massmolar massmoles of L-Histidine

moles of L-Histidine=0.24g/155.155g/mol

For Histidine HCl Monohydrate:

moles of Histidine HCl Monohydrate=mass / molar mass

moles of Histidine HCl Monohydrate=3.87 g/ 169.6 g/mol

Calculate total moles of solute:

Total moles of solute=moles of L-Histidine+moles of Histidine HCl Monohydrate

Calculate volume of solution in liters:

  • If concentrations are given in g/L, the volume in liters can be directly used as 1 L.
  • If concentrations are given in other units, convert them to g/L.

Calculate Molarity: Molarity=Total moles of solute / Volume of solution (in liters)

Just plug in the values into the formula to get the molarity of the buffer solution.

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