I have given the concentration of L-Histidine (FW: 155.155g/mol) at 0.24g/L and concentration of Histidine HCl Monohydrate (FW:169.6g/mol) at 3.87g/L. How do I calculate the molarity of buffer?
To calculate the molarity of a buffer solution, you need to follow these steps:
Convert mass to moles:
For L-Histidine:
moles of L-Histidine= molar massmass/massmolar massmoles of L-Histidine
moles of L-Histidine=0.24g/155.155g/mol
For Histidine HCl Monohydrate:
moles of Histidine HCl Monohydrate=mass / molar mass
moles of Histidine HCl Monohydrate=3.87 g/ 169.6 g/mol
Calculate total moles of solute:
Total moles of solute=moles of L-Histidine+moles of Histidine HCl Monohydrate
Calculate volume of solution in liters:
Calculate Molarity: Molarity=Total moles of solute / Volume of solution (in liters)
Just plug in the values into the formula to get the molarity of the buffer solution.