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Help regards to chemistry help forums (or with column chromatography)

It has been some time for me since college. So, I cannot remember what chemistry forums I used to seek for help. 

Currently, I need help in preparing column chromatography. My sample (of plant material) is in 100% methanol. I have analyzed the chemical activity in 0 to 10% methanol. 

I am using a c18 reversed phase silica gel. I am not sure how I should compact it into a 1.5 cm x 5 cm borosilicate glass chromatography column with PP packing reservoir funnels and end caps. According to a  chromatography paper I referenced, for plant sample analysis, 0.1% formic acid is best to use as an eluent in terms of peak shape and intensity. While methanol solutions have been tested in the paper, it did not have the best results. Currently, my goal is not to identify all plant chemicals. My goal is to fraction my plant sample into least diluted fractions as possible. Then, I am to test each elution fraction to see which fraction has the chemical activity I am looking for. 

Due to my tests in chemical activity with methanol, I am not sure if switching to formic acid, acetic acid, or other eluent is the best choice. I also do not know what wash to use so that I can reuse the column. In fact, I feel that I do not know enough about column chromatography to fully prepare a working column from scratch without wasting some materials in the process. Please let me know where I can post the question to get reliable information!

Thank you in advance!

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New Contributor II

Re: Help regards to chemistry help forums (or with column chromatography)

Chemistry help forums are a great resource for anyone seeking assistance in chemistry. They can be used to ask questions, find answers, and gain advice from experienced chemists. In particular, column chromatography is a technique that can be difficult to understand. Chemistry help forums can provide invaluable advice and support  on to those learning of Capcut for Windows or using column chromatography. They can provide guidance on different types of columns, operating procedures, and troubleshooting

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New Contributor

Re: Help regards to chemistry help forums (or with column chromatography)

Chemistry help forums serve as excellent platforms for individuals seeking assistance in the field of chemistry. These forums offer valuable resources to ask questions, discover answers, and receive advice from seasoned chemists. Specifically, the technique of column chromatography can be quite challenging to grasp. Chemistry help forums prove to be invaluable in offering guidance and support to those learning about column chromatography, as well as other topics such as inshot for pc , Free Fire for PC  , or any other related inquiries. These forums provide a wealth of information on various types of columns, operating procedures, and troubleshooting techniques, ensuring that learners can navigate the intricacies of this technique with confidence.

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