Direct Analysis of Glycosylated Therapeutics

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Direct Analysis of Glycosylated Therapeutics

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Published on ‎09-25-2023 03:40 PM by | Updated on ‎09-25-2023 03:40 PM

Glycosylation plays a crucial role in determining the pharmacological properties of biotherapeutics. The molecular heterogeneity of glycosylated biotherapeutics makes it difficult to provide thorough characterization of intact glycoproteins by mass spectrometry. Current methods for analyzing biotherapeutics only measure fragments of the compound or rely on partial digestion of the glycans to reduce sample complexity. These limitations pose important risks and challenges for biotechnology companies that need to report human-compatible and consistent glycosylation, critical for drug safety and efficacy.  
We will present a new approach to glycoform fingerprinting that uses proton-transfer charge-reduction with gas-phase fractionation to analyze intact glycoproteins by mass spectrometry. The method provides a detailed landscape of the intact molecular weights present in biotherapeutic protein preparations in a single experiment and offers insights into glycoform composition when coupled with a suitable bioinformatic strategy. In addition, we highlight the application of charge detection native mass spectrometry (Direct Mass Technology mode) towards the elucidation of masses and aggregation of heterogeneous glycosylated biotherapeutics. 
Key Learning Objectives:
  • How to elucidate masses from heterogeneous glycosylated biotheraputics 
  • Learn how proton transfer charge reduction (PTCR) can simplify complex protein mixtures 
  • Learn when to use PTCR based methods compared to Direct Mass Technology mode for analysis of biotherapeutics 
Who Should Attend:
  • Laboratory Managers 
  • Biopharmaceutical Researchers 
  • Protein Scientists 

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%7Ba1e7d76e-7411-460f-9dab-922ac63eaf1e%7D_Speaker_100x100Wendy Sandoval
Director, Translational Mass Spectrometry,

%7B2ca08302-9b4a-4f05-9567-32b5c3f9f223%7D_AnnThayer_100x100Ann Thayer
Contributing Editor,
C&EN Media Group

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