Direct Analysis of Glycosylated Therapeutics
Glycosylation plays a crucial role in determining the pharmacological properties of biotherapeutics. The molecular heterogeneity of glycosylated biotherapeutics makes it difficult to provide thorough characterization of intact glycoproteins by mass spectrometry. Current methods for analyzing biotherapeutics only measure fragments of the compound or rely on partial digestion of the glycans to reduce sample complexity. These limitations pose important risks and challenges for biotechnology companies that need to report human-compatible and consistent glycosylation, critical for drug safety and efficacy.
We will present a new approach to glycoform fingerprinting that uses proton-transfer charge-reduction with gas-phase fractionation to analyze intact glycoproteins by mass spectrometry. The method provides a detailed landscape of the intact molecular weights present in biotherapeutic protein preparations in a single experiment and offers insights into glycoform composition when coupled with a suitable bioinformatic strategy. In addition, we highlight the application of charge detection native mass spectrometry (Direct Mass Technology mode) towards the elucidation of masses and aggregation of heterogeneous glycosylated biotherapeutics.
Key Learning Objectives:
- How to elucidate masses from heterogeneous glycosylated biotheraputics
- Learn how proton transfer charge reduction (PTCR) can simplify complex protein mixtures
- Learn when to use PTCR based methods compared to Direct Mass Technology mode for analysis of biotherapeutics
Who Should Attend:
- Laboratory Managers
- Biopharmaceutical Researchers
- Protein Scientists
Brought to you by:
Speakers:
Director, Translational Mass Spectrometry,
Genentech
Contributing Editor,
C&EN Media Group
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Start:
Thu, Oct 19, 2023 12:00 PM EDT
End:
Thu, Oct 19, 2023 01:00 PM EDT
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